ELISA principals and applications for professionals

Enzyme-linked immunosorbent Assay (ELISA) ELISA from fundamentals of immune system and the history of theanalytical assay prior to the invention of ELISA to the materials of choice forfabrication of the platforms, possible biomolecular interactions, different protocols,and evaluation parameters among the rest. This book guides Readers through different steps of the analytical assay while familiarizing them with the possiblesources of error in the assay. The course offers detailed insights on the immobilization techniques used for protein attachment, different methods for evaluationof the assay and calculation of the key important parameters in the analytical assaysuch as sensitivity, specificity, accuracy, and limit of detection. Advantages andshortages of the conventional ELISA as well as different attempts for improvementof its performance are covered in this book. Merging and intergrading differenttechnologies with widely known ELISA have opened numerous windows ofopportunity to the advancement of this immunoassay. In that respect, the currentbook provides the latest updates on integrated platforms such as ELISpot, plasmonic ELISA, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs as well asELISA in micro-devices. Fundamentals and History of ELISA Evolution of the Immunoassays Until Invention of ELISA Principles of the Immune System Biomolecular Interactions Between Antibody and Antigen . . .General Overviews on Applications of ELISA . . .Step by Step with ELISA: Mechanism of Operation, CrucialElements, Different Protocols, and Insights on Immobilizationand Detection of Various Biomolecular Entities . . . . . . . . . Evaluation of the Detection Results Obtained from ELISA . . . .